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  • Benny Miles posted an update 1 year, 6 months ago

    Although plasma acylated ghrelin levels in aged male mice exposed to novelty stress were significantly lower at 3?h and 6?h after exposure than in aged control mice, levels did not change in aged female mice (Fig. 5A and B). The decreased cumulative food intake in aged male mice at 1�C3?h after exposure to novelty stress was completely inhibited on IP administration of 10?nmol/kg exogenous acylated ghrelin (Fig. 5C). Two-way factorial ANOVA revealed no significant effect of stress [F(1,14)?=?3.51, n.s.], treatment selleck chemicals [F(1,14)?=?3.73, n.s.], or stress?��?treatment interaction [F(1,14)?=?3.20, n.s.] at 1?h; a significant effect of treatment [F(1,14)?=?6.33, p?<?0.05]; no significant effect of stress [F(1,14)?=?2.71, n.s.] or stress?��?treatment interaction [F(1,14)?=?4.02, n.s.] at 3?h; no significant effect of stress [F(1,14)?=?2.45, n.s.] or treatment [F(1,14)?=?3.35, n.s.]; and a significant effect of stress?��?treatment interaction [F(1,14)?=?4.75, p?<?0.05] at 6?h. The decrease in plasma acylated ghrelin levels in aged male mice exposed to novelty stress was blocked by administering the 5-HT2CR antagonists SB242084 and RKT ( Fig. 5D). The plasma acylated ghrelin levels in aged female mice exposed to novelty stress were unaffected by the administration of SB242084 (data not shown). To clarify gender differences in food intake in aged mice exposed to novelty stress, we focused on hypothalamic ER��, ER��, and aromatase mRNA expression levels. Compared Roxadustat solubility dmso with young male mice, hypothalamic ER�� and aromatase mRNA expression levels significantly increased in aged male mice, whereas ER�� mRNA expression levels remained unaltered in aged mice. In contrast, in comparison with young female mice, ER�� and aromatase mRNA expression PTPRJ levels in aged female mice tended to decrease, but ER�� mRNA expression levels were not altered (Table 1). Next, we examined the role of aromatase and ER�� on the decreased food intake in aged male mice exposed to novelty stress. As observed in Fig. 5E, oral administration of letrozole to aged male mice significantly inhibited novelty stress-induced decrease in food intake by 81% at 6?h after the exposure. In contrast, in aged female mice, letrozole administration did not significantly increase food intake after novelty stress exposure compared with control mice. SC administration of the selective ER�� agonist DPN had no effects on novelty stress-induced hypophagia in aged male or female mice. Administration of selective ER�� agonist PPT at 1.25?mg/kg did not decrease food intake in young male and female mice compared with that in control mice (Fig. 5F and G). Furthermore, in aged male mice, treatment with the same or much lower doses of PPT (0.63 and 1.25?mg/kg) suppressed food intake by 28�C76% at 6?h and 24?h after administration. Two-way factorial ANOVA revealed significant effects of treatment [F(2,19)?=?19.68, p?<?0.001] and treatment?��?age interaction [F(2,19)?=?5.17, p?<?0.